Antifungal activity of recombinant mouse beta-defensin 3
نویسندگان
چکیده
منابع مشابه
Mouse beta-defensin-14, an antimicrobial ortholog of human beta-defensin-3.
Searching the database for mouse homologs of the antimicrobial peptide human beta-defensin-3 (hBD-3) revealed highest identity (69%) to mouse beta-defensin-14 (mBD-14). Recombinant mBD-14 exhibited broad-spectrum, nanomolar microbicidal activity. Treatment of keratinocytes with gamma interferon or transforming growth factor alpha increased mBD-14 gene expression. These data suggest that mBD-14 ...
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Plant lipid transfer proteins (LTPs) are members of the pathogenesis-related proteins (PR-14) and some of them exhibit activity against phytopathogenic fungi. To investigate whether rice LTP2 plays a role in antifungal activity, the coding region of an Iranian rice Ltp2 gene was cloned into expression vector pET24-d(+) and then expressed in Escherichia coli Rosetta strain (DE3). The potential a...
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OBJECTIVES The purpose of this ex vivo study was to compare the antifungal activity of a synthetic peptide consisting of 15 amino acids at the C-terminus of human β-defensin 3 (HBD3-C15) with calcium hydroxide (CH) and Nystatin (Nys) against Candida albicans (C. albicans) biofilm. MATERIALS AND METHODS C. albicans were grown on cover glass bottom dishes or human dentin disks for 48 hr, and th...
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Mature mouse beta defensin 2 (mBD2) is a small cationic peptide with antimicrobial activity. Here we established a prokaryotic expression vector containing the cDNA of mature mBD2 fused with thioredoxin (TrxA), pET32a-mBD2. The vector was transformed into Escherichia Coli (E. coli) Rosseta-gami (2) for expression fusion protein. Under the optimization of fermentation parameters: induce with 0.6...
متن کاملHuman beta-defensin 2 and 3 and their mouse orthologs induce chemotaxis through interaction with CCR2.
Beta-defensins play a dual role during immune response. Their direct antimicrobial properties contribute to the local innate immune response by combating microbial invasions. Furthermore, previous studies revealed the capacity of certain beta-defensin family members to chemoattract immature dendritic cells and CD45RO+ CD4+ T cells through chemokine receptor CCR6. However, because beta-defensins...
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ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2010
ISSN: 0266-8254,1472-765X
DOI: 10.1111/j.1472-765x.2010.02824.x